WaterTight
Back to Knowledge Base
Sampling

How to Take a TVC (Bacteria Count) Sample

How to collect water samples to check how many bacteria are in the water.

What is a TVC?

TVC stands for Total Viable Count. It tells you how many bacteria are in the water. It is a general check of how clean the water system is.

How to Take the Sample

  1. Use a clean 500ml or 1 litre bottle (with sodium thiosulphate if the water is chlorinated).
  2. Remove any attachments from the tap (aerators, shower heads).
  3. Do NOT clean the tap - you want to see the real condition.
  4. Run the water for 2-3 seconds only (do NOT flush for a long time).
  5. Fill the bottle without touching the tap.
  6. Label it with date, time, location, and tap name.
  7. Keep at 2-8 degrees C in a cool box.
  8. Get it to the lab within 24 hours.

What the Results Mean

The lab grows the bacteria at two temperatures:

At 22 degrees C (bugs from the environment)

| Result | Meaning | |---|---| | Under 100 CFU/ml | Good | | 100-1,000 CFU/ml | Check the system | | Over 1,000 CFU/ml | Bad - investigate and fix |

At 37 degrees C (bugs from people/animals)

| Result | Meaning | |---|---| | Under 10 CFU/ml | Good | | 10-100 CFU/ml | Check the system | | Over 100 CFU/ml | Bad - investigate and fix |

What to Do If Results Are Bad

  1. Look for where the bacteria are coming from.
  2. Check temperatures are right.
  3. Clean and disinfect if needed.
  4. Test again 2-7 days after fixing.
  5. If still bad, do a full investigation.

Warnings & Safety

Do NOT flush the tap before sampling - this gives a false low result. Samples must reach the lab within 24 hours at 2-8 degrees C.

Relevant Legislation

HSG274 Part 2, ACOP L8 (4th Edition)