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Sampling
How to Take a TVC (Bacteria Count) Sample
How to collect water samples to check how many bacteria are in the water.
What is a TVC?
TVC stands for Total Viable Count. It tells you how many bacteria are in the water. It is a general check of how clean the water system is.
How to Take the Sample
- Use a clean 500ml or 1 litre bottle (with sodium thiosulphate if the water is chlorinated).
- Remove any attachments from the tap (aerators, shower heads).
- Do NOT clean the tap - you want to see the real condition.
- Run the water for 2-3 seconds only (do NOT flush for a long time).
- Fill the bottle without touching the tap.
- Label it with date, time, location, and tap name.
- Keep at 2-8 degrees C in a cool box.
- Get it to the lab within 24 hours.
What the Results Mean
The lab grows the bacteria at two temperatures:
At 22 degrees C (bugs from the environment)
| Result | Meaning | |---|---| | Under 100 CFU/ml | Good | | 100-1,000 CFU/ml | Check the system | | Over 1,000 CFU/ml | Bad - investigate and fix |
At 37 degrees C (bugs from people/animals)
| Result | Meaning | |---|---| | Under 10 CFU/ml | Good | | 10-100 CFU/ml | Check the system | | Over 100 CFU/ml | Bad - investigate and fix |
What to Do If Results Are Bad
- Look for where the bacteria are coming from.
- Check temperatures are right.
- Clean and disinfect if needed.
- Test again 2-7 days after fixing.
- If still bad, do a full investigation.
Warnings & Safety
Do NOT flush the tap before sampling - this gives a false low result. Samples must reach the lab within 24 hours at 2-8 degrees C.
Relevant Legislation
HSG274 Part 2, ACOP L8 (4th Edition)
